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Shake Test

What is "Shake test”?
Shake test is used for determining if an adsorbed vaccine has been frozen anytime in the past. The test is valid for "adsorbed vaccines” only, such as DPT, DT, TT, Pentavalent vaccine and Hepatitis-B.

DPT AND TT VACCINES

Diphtheria, Pertussis, and Tetanus(DPT; Triple Antigen)

Identification features
The vaccine is available in small glass bottles mostly having a blue metallic cover.

Type of vaccine
Killed (diphtheria toxoid, tetanus toxoid, and killed bacteria of pertussis)

Site of injection
Anterolateral aspect of LEFT mid-thigh

IPV (INACTIVATED POLIOVIRUS VACCINE)

Type of Vaccine
Inactivated polio virus (killed virus)

COMPOSITION
Inactivated poliovirus type 1 - 40 D antigen units
Inactivated poliovirus type 2 - 8 D antigen units
Inactivated poliovirus type 3 - 32 D antigen units

Number of doses per vial
Vaccine comes in: 1-dose, 5-dose, and 10-dose vials
The Government of India is acquiring 5-dose and 10-dose vials currently

Type of vaccine vial monitor (VVM) on the vial
Type 7

PENTAVALENT VACCINE

Type of Vaccine
Killed
Liquid form (liquid pentavalent vaccine) is available under UIP.

COMPOSITION
Each dose of 0.5 ml contains
1. Diphtheria Toxoid
2. Tetanus Toxoid
3. B. pertussis (whole cell)
4. HBsAg (rDNA)
5. Purified capsular Hib Polysaccharide (PRP)

Number of doses per vial
The liquid pentavalent vaccine (LPV) in the UIP (Universal Immunization Program, India) is available as a multi-dose vial with 10 doses per vial.

BACILLE CALMETTE GUERIN (BCG) VACCINE

Identification features of BCG vaccine (Fig. 4.1)
• The vaccine is available in dark bottles to protect from sunlight.
• Some bottles have a long neck as the vaccine is packed in vacuum.

Type of vaccine
• Live
• Freeze dried powder

Dose
For institutional deliveries: 0.05 mL at the time of birth or up to age of 4 weeks
• In case of home delivery, if given at 6 weeks: 0.1 mL (along with Pentavalent – 1 and other routine vaccines)

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